PCR & qPCR (amplification)
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Knowledge and insights about PCR & qPCR (amplification) from the Science Hub
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Having trouble when amplifying GC-rich sequences? Here are four tips on how to troubleshoot
GC-rich templates in DNA sequences have 60% or more G (guanine) or C (cytosine) bases. Amplifying GC-rich templates may require adjusting reagents, annealing temperature, and using specialized master mixes or standalone polymerases for optimal results, and in this blog article you get four tips on how to troubleshot.
Read moreHow to evaluate your qPCR and RT-qPCR results
qPCR and RT-qPCR methods are described, their usefulness and power as well as their sensitivity and vulnerability. In addition, it is explained how qPCR evaluation is accomplished by applying the “MIQE” guidelines and NEB´s “dots in boxes” quality scoring methods.
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Tomato leaves SlChr4-NC1 primer pair
Diagenode
Tomato leaves SlChr4-NC1 primer pair - 50 µl
Tomato leaves SlChr4-NC1 primer pair
Diagenode
Tomato leaves SlChr4-NC1 primer pair - 500 µl
Tomato leaves SlChr2-reg8 primer pair
Diagenode
Tomato leaves SlChr2-reg8 primer pair - 500 µl
Tomato leaves SlChr2-reg8 primer pair
Diagenode
Tomato leaves SlChr2-reg8 primer pair - 50 µl
Rice seedlings OsMADS6 primer pair
Diagenode
Rice seedlings OsMADS6 primer pair - 50 µl
Rice seedlings OsMADS6 primer pair
Diagenode
Rice seedlings OsMADS6 primer pair - 500 µl
Rice seedlings OsChr4-reg9 primer pair
Diagenode
Rice seedlings OsChr4-reg9 primer pair - 50 µl
Rice seedlings OsChr4-reg9 primer pair
Diagenode
Rice seedlings OsChr4-reg9 primer pair - 500 µl
Rat TSH2B coding region primer pair
Diagenode
Rat TSH2B coding region primer pair - 50 µl
Rat TSH2B coding region primer pair
Diagenode
Rat TSH2B coding region primer pair - 500 µl
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